Hotspot

ndhF-rpl32

SSC · 133032-133977 · 946 bp

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Pi 0.44
MarkerSeek score 81.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
946
variable_sites
362
pi
0.44
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
81.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGGGAATTGGGCAGACTTAG AGCGTGTCTACCGATTTCAC 55.0 50.0 57.9 58.0 2340 bp 0.5 59.5
ndhF-rpl32_p2 GGGGAATTGGGCAGACTTAG CAGCGTGTCTACCGATTTCA 55.0 50.0 57.9 58.0 2341 bp 0.5 59.5
ndhF-rpl32_p3 AAATAACAGGAAGGCGCACA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2307 bp 0.5 59.2
ndhF-rpl32_p4 AATAACAGGAAGGCGCACAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2306 bp 0.5 59.2
ndhF-rpl32_p5 ATAACAGGAAGGCGCACAAA AGCGTGTCTACCGATTTCAC 45.0 50.0 58.1 58.0 2305 bp 0.5 59.2