Hotspot

psaJ-rpl33

LSC · 73536-74085 · 550 bp

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Pi 0.0168
MarkerSeek score 69.80
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
550
variable_sites
9
pi
0.0168
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
69.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 GTGGCACCAGTGGTAAGTAC ACACTGTTTCGAACACAATTGG 55.0 40.9 58.2 58.0 726 bp 1.0 64.8
psaJ-rpl33_p2 GTGGCACCAGTGGTAAGTAC CACTGTTTCGAACACAATTGGT 55.0 40.9 58.2 58.0 725 bp 1.0 64.8
psaJ-rpl33_p3 GTGGCACCAGTGGTAAGTAC TCGGATATCTTTACCTTTGGCC 55.0 45.5 58.2 57.9 682 bp 1.0 64.5
psaJ-rpl33_p4 TGGCACCAGTGGTAAGTACT ACACTGTTTCGAACACAATTGG 50.0 40.9 58.3 58.0 725 bp 1.0 64.1
psaJ-rpl33_p5 TGGCACCAGTGGTAAGTACT CACTGTTTCGAACACAATTGGT 50.0 40.9 58.3 58.0 724 bp 1.0 64.1