Hotspot

psaA-ycf3

LSC · 46641-47465 · 825 bp

Back to Inga

Pi 0.0297
MarkerSeek score 67.90
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
825
variable_sites
22
pi
0.0297
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.90
markerseek_score
67.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 CCCATTCCTCGAAAGACGTT TTGGTTGAAGATCACAGGGC 50.0 50.0 57.9 58.1 908-935 bp 1.0 84.4
psaA-ycf3_p2 AGAAATGACCGGGTTTAGCC TTGGTTGAAGATCACAGGGC 50.0 50.0 57.9 58.1 926-953 bp 1.0 84.2
psaA-ycf3_p3 CCCATTCCTCGAAAGACGTT AAGATCACAGGGCGTTTTGA 50.0 45.0 57.9 57.7 901-928 bp 1.0 83.0
psaA-ycf3_p4 CCCATTCCTCGAAAGACGTT AGATCACAGGGCGTTTTGAA 50.0 45.0 57.9 57.7 900-927 bp 1.0 83.0
psaA-ycf3_p5 CCCATTCCTCGAAAGACGTT TGAAGATCACAGGGCGTTTT 50.0 45.0 57.9 57.7 903-930 bp 1.0 83.0