Hotspot

ndhG

SSC · 113349-113879 · 531 bp

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Pi 0

MarkerSeek score 36.90

Primer available yes

Label ndhG

Feature Metadata

label_name: ndhG
feature_type: gene
region: SSC
length_bp: 531
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 36.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG_p1	TGTTGGCAGTAGATGGGTTT	TCCCTTTTTCCTTCCTTGAATCT	45.0	39.1	57.0	57.7	710-721 bp	1.0	46.8
ndhG_p2	TTGTTGGCAGTAGATGGGTT	TCCCTTTTTCCTTCCTTGAATCT	45.0	39.1	57.0	57.7	711-722 bp	1.0	46.8
ndhG_p3	TTTGTTGGCAGTAGATGGGT	TCCCTTTTTCCTTCCTTGAATCT	45.0	39.1	57.0	57.7	712-723 bp	1.0	46.8
ndhG_p4	TGTTGGCAGTAGATGGGTTT	TCCTAATCCCTTTTTCCTTCCT	45.0	40.9	57.0	56.4	716-727 bp	1.0	44.7
ndhG_p5	TTGTTGGCAGTAGATGGGTT	TCCTAATCCCTTTTTCCTTCCT	45.0	40.9	57.0	56.4	717-728 bp	1.0	44.7