Hotspot

rpl32-trnL-UAG

SSC · 108462-109163 · 702 bp

Back to Indosasa

Pi 0.00351
MarkerSeek score 49.90
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
702
variable_sites
7
pi
0.00351
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AAGGGTTTTTCTGGGCAACA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 774-778 bp 1.0 78.7
rpl32-trnL-UAG_p2 AGGGTTTTTCTGGGCAACAA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.1 57.9 773-777 bp 1.0 78.7
rpl32-trnL-UAG_p3 AAGATCATTTTCCAGCGGCA TCCTAAGAGCAGCGTGTCTA 45.0 50.0 58.2 57.9 811-815 bp 1.0 78.4
rpl32-trnL-UAG_p4 AAGGGTTTTTCTGGGCAACA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 767-771 bp 1.0 78.2
rpl32-trnL-UAG_p5 AGGGTTTTTCTGGGCAACAA AGCAGCGTGTCTACCAATTT 45.0 45.0 58.1 57.8 766-770 bp 1.0 78.2