Hotspot

ndhF-rpl32

SSC · 107415-108281 · 867 bp

Back to Indosasa

Pi 0.000866

MarkerSeek score 50.30

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 867
variable_sites: 3
pi: 0.000866
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGAAGTGGGAGAAGAGGGAT	TCCAAAGATTTTTACGAATACGCT	55.0	33.3	58.1	57.3	977-1007 bp	1.0	41.6
ndhF-rpl32_p2	GGAAGTGGGAGAAGAGGGAT	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	949-979 bp	1.0	41.3
ndhF-rpl32_p3	GGAAGTGGGAGAAGAGGGAT	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	950-980 bp	1.0	41.3
ndhF-rpl32_p4	GGAAGTGGGAGAAGAGGGAT	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	951-981 bp	1.0	41.3
ndhF-rpl32_p5	GGAAGTGGGAGAAGAGGGAT	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	952-982 bp	1.0	41.3