Hotspot

petG-trnW-CCA

LSC · 66415-66536 · 122 bp

Back to Indosasa

Pi 0.0184

MarkerSeek score 53.60

Primer available yes

Label petG-trnW-CCA

Feature Metadata

label_name: petG-trnW-CCA
feature_type: igs
region: LSC
length_bp: 122
variable_sites: 9
pi: 0.0184
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petG-trnW-CCA_p1	TACAGACGTGGGGATCAGTT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	227 bp	1.0	82.5
petG-trnW-CCA_p2	TACAGACGTGGGGATCAGTT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	191 bp	1.0	82.5
petG-trnW-CCA_p3	GGGATTATTCGTGACTGCGT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	58.1	58.0	255 bp	1.0	82.2
petG-trnW-CCA_p4	GGGATTATTCGTGACTGCGT	CAAAACCCGATGTCGTAGGT	50.0	50.0	58.1	57.9	219 bp	1.0	82.1
petG-trnW-CCA_p5	ATACAGACGTGGGGATCAGT	AGCGCTCTTAGTTCAGTTCG	50.0	50.0	57.8	58.0	228 bp	1.0	81.7