Hotspot

psbM-petN

LSC · 18697-19468 · 772 bp

Back to Indosasa

Pi 0.00185

MarkerSeek score 57.90

Primer available yes

Label psbM-petN

Feature Metadata

label_name: psbM-petN
feature_type: igs
region: LSC
length_bp: 772
variable_sites: 5
pi: 0.00185
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 57.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-petN_p1	TGGAAGTCAATATTCTCGCA	TAGTAAGTCTCGCTTGGGCT	40.0	50.0	54.5	58.2	941-998 bp	1.0	52.6
psbM-petN_p2	TGGAAGTCAATATTCTCGCA	GTAGTATGGGGGAGGAGTGG	40.0	60.0	54.5	58.3	885-942 bp	1.0	51.6
psbM-petN_p3	TGGAAGTCAATATTCTCGCA	TAGTATGGGGGAGGAGTGGA	40.0	55.0	54.5	58.4	884-941 bp	1.0	50.9
psbM-petN_p4	TGCTACTGCACTGTTTATTCT	TAGTAAGTCTCGCTTGGGCT	38.1	50.0	55.2	58.2	916-973 bp	1.0	50.7
psbM-petN_p5	TGCTACTGCACTGTTTATTCT	GTAGTATGGGGGAGGAGTGG	38.1	60.0	55.2	58.3	860-917 bp	1.0	49.8