Hotspot

trnG-UCC-trnT-GGU

LSC · 14352-16352 · 2001 bp

Back to Indosasa

Pi 0.001
MarkerSeek score 53.30
Primer available yes
Label trnG-UCC-trnT-GGU

Feature Metadata

label_name
trnG-UCC-trnT-GGU
feature_type
igs
region
LSC
length_bp
2001
variable_sites
8
pi
0.001
unique_haplotype_count
7
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnT-GGU_p1 ACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 38.1 52.4 55.5 58.1 2129-2155 bp 1.0 42.9
trnG-UCC-trnT-GGU_p2 ACGAATCACACTTTTACCACT GACTTACGTCTTACCATGGCA 38.1 47.6 55.5 57.8 2142-2168 bp 1.0 42.2
trnG-UCC-trnT-GGU_p3 ACGAATCACACTTTTACCACT TGACTTACGTCTTACCATGGC 38.1 47.6 55.5 57.8 2143-2169 bp 1.0 42.2
trnG-UCC-trnT-GGU_p4 AGAACGAATCACACTTTTACCAC CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2132-2158 bp 1.0 41.9
trnG-UCC-trnT-GGU_p5 GAACGAATCACACTTTTACCACT CCATGGCATTACTCTACCACC 39.1 52.4 57.3 58.1 2131-2157 bp 1.0 41.9