Hotspot

ycf3

LSC · 36681-38667 · 1987 bp

Back to Indocalamus

Pi 0.000112

MarkerSeek score 50.00

Primer available yes

Label ycf3

Feature Metadata

label_name: ycf3
feature_type: gene
region: LSC
length_bp: 1987
variable_sites: 1
pi: 0.000112
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf3_p1	AGAGCATATGGATCGATCGA	ATCAACAACCAAACCACCCA	45.0	45.0	55.7	57.8	2131-2144 bp	1.0	60.7
ycf3_p2	AGAGCATATGGATCGATCGA	TCAACAACCAAACCACCCAT	45.0	45.0	55.7	57.8	2130-2143 bp	1.0	60.7
ycf3_p3	AGAGCATATGGATCGATCGAT	ATCAACAACCAAACCACCCA	42.9	45.0	56.3	57.8	2131-2144 bp	1.0	57.2
ycf3_p4	AGAGCATATGGATCGATCGAT	TCAACAACCAAACCACCCAT	42.9	45.0	56.3	57.8	2130-2143 bp	1.0	57.2
ycf3_p5	AGAGCATATGGATCGATCGA	GATCAACAACCAAACCACCC	45.0	50.0	55.7	57.2	2132-2145 bp	1.0	55.5