Hotspot

cemA

LSC · 21287-21979 · 693 bp

Back to Indocalamus

Pi 0.000641

MarkerSeek score 44.80

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 693
variable_sites: 2
pi: 0.000641
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 44.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	TGCTAAAATGGAAAAGGGCT	GAGTCAGCGAATGAAGCAGA	40.0	50.0	55.5	58.0	819 bp	1.0	60.1
cemA_p2	TGCTAAAATGGAAAAGGGCT	AGAGTCAGCGAATGAAGCAG	40.0	50.0	55.5	58.0	820 bp	1.0	60.0
cemA_p3	TGCTAAAATGGAAAAGGGCTT	GAGTCAGCGAATGAAGCAGA	38.1	50.0	56.3	58.0	819 bp	1.0	58.5
cemA_p4	TTGCTAAAATGGAAAAGGGCT	GAGTCAGCGAATGAAGCAGA	38.1	50.0	56.3	58.0	820 bp	1.0	58.5
cemA_p5	TGCTAAAATGGAAAAGGGCTT	AGAGTCAGCGAATGAAGCAG	38.1	50.0	56.3	58.0	820 bp	1.0	58.5