Hotspot

trnQ-UUG-rps16

LSC · 75990-77535 · 1546 bp

Back to Indocalamus

Pi 0.00199

MarkerSeek score 34.20

Primer available yes

Label trnQ-UUG-rps16

Feature Metadata

label_name: trnQ-UUG-rps16
feature_type: igs
region: LSC
length_bp: 1546
variable_sites: 9
pi: 0.00199
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 34.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-rps16_p1	GAGGTTCGAATCCTTCCGTC	AAGTCGCACGTTGCTTTCTA	55.0	45.0	58.1	58.1	1604-1622 bp	1.0	80.0
trnQ-UUG-rps16_p2	GAGGTTCGAATCCTTCCGTC	TCATGTCCTTCAAGTCGCAC	55.0	50.0	58.1	58.2	1615-1633 bp	1.0	79.4
trnQ-UUG-rps16_p3	CTCGGAGGTTCGAATCCTTC	AAGTCGCACGTTGCTTTCTA	55.0	45.0	57.8	58.1	1608-1626 bp	1.0	78.9
trnQ-UUG-rps16_p4	CTCGGAGGTTCGAATCCTTC	TCATGTCCTTCAAGTCGCAC	55.0	50.0	57.8	58.2	1619-1637 bp	1.0	78.3
trnQ-UUG-rps16_p5	TAAAACCAGAAATGGGGCGT	AAGTCGCACGTTGCTTTCTA	45.0	45.0	57.7	58.1	1664-1682 bp	1.0	78.1