Hotspot

trnfM-CAU-trnG-UCC

LSC · 69677-70124 · 448 bp

Back to Indocalamus

Pi 0.00292

MarkerSeek score 50.00

Primer available yes

Label trnfM-CAU-trnG-UCC

Feature Metadata

label_name: trnfM-CAU-trnG-UCC
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 5
pi: 0.00292
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 50.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnfM-CAU-trnG-UCC_p1	CGAGGCTCATAACCTTGAGG	GCGAGCGTAGTTCAATGGTA	55.0	50.0	57.8	58.1	551-566 bp	1.0	81.1
trnfM-CAU-trnG-UCC_p2	CAGTTTGGTAGCTCACGAGG	GCGAGCGTAGTTCAATGGTA	55.0	50.0	58.3	58.1	566-581 bp	1.0	80.5
trnfM-CAU-trnG-UCC_p3	CGAGGCTCATAACCTTGAGG	CGTAGCGAGCGTAGTTCAAT	55.0	50.0	57.8	58.2	555-570 bp	1.0	80.3
trnfM-CAU-trnG-UCC_p4	CGAGGCTCATAACCTTGAGG	CCCAGGCGGATATACCCTAT	55.0	55.0	57.8	57.8	586-601 bp	1.0	80.1
trnfM-CAU-trnG-UCC_p5	CGAGGCTCATAACCTTGAGG	TAGCGAGCGTAGTTCAATGG	55.0	50.0	57.8	57.8	553-568 bp	1.0	80.1