Hotspot

trnT-GGU-trnfM-CAU

LSC · 66794-69602 · 2809 bp

Back to Indocalamus

Pi 0.00214
MarkerSeek score 47.50
Primer available yes
Label trnT-GGU-trnfM-CAU

Feature Metadata

label_name
trnT-GGU-trnfM-CAU
feature_type
igs
region
LSC
length_bp
2809
variable_sites
21
pi
0.00214
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-trnfM-CAU_p1 CCATGGCATTACTCTACCACC CCTCAAGGTTATGAGCCTCG 52.4 55.0 58.1 57.8 2945-2971 bp 1.0 70.0
trnT-GGU-trnfM-CAU_p2 CCATGGCATTACTCTACCACC CCTCGTGAGCTACCAAACTG 52.4 55.0 58.1 58.3 2930-2956 bp 1.0 69.6
trnT-GGU-trnfM-CAU_p3 GACTTACGTCTTACCATGGCA CCTCAAGGTTATGAGCCTCG 47.6 55.0 57.8 57.8 2958-2984 bp 1.0 69.3
trnT-GGU-trnfM-CAU_p4 TGACTTACGTCTTACCATGGC CCTCAAGGTTATGAGCCTCG 47.6 55.0 57.8 57.8 2959-2985 bp 1.0 69.3
trnT-GGU-trnfM-CAU_p5 GACTTACGTCTTACCATGGCA CCTCGTGAGCTACCAAACTG 47.6 55.0 57.8 58.3 2943-2969 bp 1.0 68.9