Hotspot

petN-psbM

LSC · 63696-64449 · 754 bp

Back to Indocalamus

Pi 0.00169

MarkerSeek score 50.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 754
variable_sites: 5
pi: 0.00169
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	TAGTAAGTCTCGCTTGGGCT	TGGAAGTCAATATTCTCGCA	50.0	40.0	58.2	54.5	936-941 bp	1.0	51.6
petN-psbM_p2	GTAGTATGGGGGAGGAGTGG	TGGAAGTCAATATTCTCGCA	60.0	40.0	58.3	54.5	880-885 bp	1.0	50.6
petN-psbM_p3	TAGTATGGGGGAGGAGTGGA	TGGAAGTCAATATTCTCGCA	55.0	40.0	58.4	54.5	879-884 bp	1.0	49.9
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	TGCTACTGCACTGTTTATTCT	50.0	38.1	58.2	55.2	911-916 bp	1.0	49.7
petN-psbM_p5	GTAGTATGGGGGAGGAGTGG	TGCTACTGCACTGTTTATTCT	60.0	38.1	58.3	55.2	855-860 bp	1.0	48.7