Hotspot

atpH-atpI

LSC · 48533-49364 · 832 bp

Back to Indocalamus

Pi 0.0017

MarkerSeek score 51.70

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 832
variable_sites: 5
pi: 0.0017
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 51.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	GCAGTACCTTGACCAACTCC	TAGCCGCAGCCTATATAGGT	55.0	50.0	58.2	57.5	950-968 bp	1.0	76.2
atpH-atpI_p2	AAGCAGCAGCAATTAGTGGA	TAGCCGCAGCCTATATAGGT	45.0	50.0	57.8	57.5	883-901 bp	1.0	76.2
atpH-atpI_p3	GCCAATCCAGCAGCAATAAC	TAGCCGCAGCCTATATAGGT	50.0	50.0	57.8	57.5	905-923 bp	1.0	76.1
atpH-atpI_p4	ATAACGGAAGCAGCAGCAAT	TAGCCGCAGCCTATATAGGT	45.0	50.0	58.2	57.5	890-908 bp	1.0	75.9
atpH-atpI_p5	GCAGTACCTTGACCAACTCC	ATTTTTGCAACGTTAGCCGC	55.0	45.0	58.2	58.6	963-981 bp	1.0	75.7