Hotspot

trnL-UAA-trnT-UGU

LSC · 33926-34741 · 816 bp

Back to Indocalamus

Pi 0.000817
MarkerSeek score 49.00
Primer available yes
Label trnL-UAA-trnT-UGU

Feature Metadata

label_name
trnL-UAA-trnT-UGU
feature_type
igs
region
LSC
length_bp
816
variable_sites
3
pi
0.000817
unique_haplotype_count
6
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnL-UAA-trnT-UGU_p1 ACAATCAAGTCCGTAGCGTC ATTTGAACCGATGACCCTCG 50.0 50.0 58.0 58.0 917-942 bp 1.0 80.3
trnL-UAA-trnT-UGU_p2 ACAATCAAGTCCGTAGCGTC CGATGACCCTCGCATTACAA 50.0 50.0 58.0 58.1 909-934 bp 1.0 79.9
trnL-UAA-trnT-UGU_p3 ACAATCAAGTCCGTAGCGTC GCGATGCTCTAACCTCTGAG 50.0 55.0 58.0 57.9 887-912 bp 1.0 79.4
trnL-UAA-trnT-UGU_p4 CCGTAGCGTCTACCGATTTC ATTTGAACCGATGACCCTCG 55.0 50.0 58.2 58.0 907-932 bp 1.0 78.3
trnL-UAA-trnT-UGU_p5 CAATCAAGTCCGTAGCGTCT ATTTGAACCGATGACCCTCG 50.0 50.0 57.7 58.0 916-941 bp 1.0 78.1