Hotspot

ndhJ-trnF-GAA

LSC · 32296-32880 · 585 bp

Back to Indocalamus

Pi 0.00325

MarkerSeek score 48.10

Primer available yes

Label ndhJ-trnF-GAA

Feature Metadata

label_name: ndhJ-trnF-GAA
feature_type: igs
region: LSC
length_bp: 585
variable_sites: 7
pi: 0.00325
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 48.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhJ-trnF-GAA_p1	TGAAAGTTGGATAGGCTGGC	CCTCGTGTCACCAGTTCAAA	50.0	50.0	57.9	58.1	693-695 bp	1.0	79.7
ndhJ-trnF-GAA_p2	ATAGGCTGGCCCTTACGTAA	CCTCGTGTCACCAGTTCAAA	50.0	50.0	58.2	58.1	683-685 bp	1.0	79.1
ndhJ-trnF-GAA_p3	ATCATCCACGCCTTAAACGT	CCTCGTGTCACCAGTTCAAA	45.0	50.0	57.6	58.1	724-726 bp	1.0	77.5
ndhJ-trnF-GAA_p4	TGAAAGTTGGATAGGCTGGC	AATCCTCGTGTCACCAGTTC	50.0	50.0	57.9	57.5	696-698 bp	1.0	76.5
ndhJ-trnF-GAA_p5	GAAAGTTGGATAGGCTGGCC	CCTCGTGTCACCAGTTCAAA	55.0	50.0	58.6	58.1	692-694 bp	1.0	75.9