Hotspot

psaI-rbcL

LSC · 23411-24718 · 1308 bp

Back to Indocalamus

Pi 0.00384

MarkerSeek score 38.70

Primer available yes

Label psaI-rbcL

Feature Metadata

label_name: psaI-rbcL
feature_type: igs
region: LSC
length_bp: 1308
variable_sites: 14
pi: 0.00384
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 38.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-rbcL_p1	CCATTGCAATTGCCGGAAAT	GGCTTCAGGGGGTATTCATG	45.0	55.0	58.0	58.0	1660-1682 bp	1.0	81.6
psaI-rbcL_p2	CCATTGCAATTGCCGGAAAT	CGCCGGTACAGTAGTAGGTA	45.0	55.0	58.0	58.0	1810-1832 bp	1.0	81.3
psaI-rbcL_p3	CCATTGCAATTGCCGGAAAT	GTTATACCGGTGGCTTCAGG	45.0	55.0	58.0	58.1	1671-1693 bp	1.0	81.3
psaI-rbcL_p4	CCATTGCAATTGCCGGAAAT	CTTAACTGGGGGATTCACCG	45.0	55.0	58.0	58.0	1984-2006 bp	1.0	81.2
psaI-rbcL_p5	CCAAGATAACTGGAAGCCCC	GGCTTCAGGGGGTATTCATG	55.0	55.0	57.9	58.0	2198-2220 bp	1.0	81.0