Hotspot

ndhG-ndhI

SSC · 123993-124604 · 612 bp

Back to Indigofera

Pi 0.0496

MarkerSeek score 86.10

Primer available yes

Label ndhG-ndhI

Feature Metadata

label_name: ndhG-ndhI
feature_type: igs
region: SSC
length_bp: 612
variable_sites: 119
pi: 0.0496
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 86.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhG-ndhI_p1	CAAGACCCGATCCCAGAAAG	AATCAAATTGCTTTGGGCCG	55.0	45.0	58.0	57.9	674-791 bp	1.0	91.0
ndhG-ndhI_p2	AAGACCCGATCCCAGAAAGA	AATCAAATTGCTTTGGGCCG	50.0	45.0	58.0	57.9	673-790 bp	1.0	90.8
ndhG-ndhI_p3	TACAAGACCCGATCCCAGAA	AATCAAATTGCTTTGGGCCG	50.0	45.0	57.8	57.9	676-793 bp	1.0	89.3
ndhG-ndhI_p4	CAAGACCCGATCCCAGAAAG	TTTGGGCCGTTTACCAATGT	55.0	45.0	58.0	58.3	663-780 bp	0.913043	85.9
ndhG-ndhI_p5	AAGACCCGATCCCAGAAAGA	TTTGGGCCGTTTACCAATGT	50.0	45.0	58.0	58.3	662-779 bp	0.913043	85.8