Hotspot

rpl32-trnL-UAG

SSC · 118423-119380 · 958 bp

Back to Indigofera

Pi 0.0706
MarkerSeek score 75.30
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
958
variable_sites
226
pi
0.0706
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.71
markerseek_score
75.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 AGAGGGGATATTGGAAAGCA GAAGTAGCAAAGCCTTGGGT 45.0 50.0 55.9 58.1 1124-1578 bp 0.956522 72.5
rpl32-trnL-UAG_p2 AAAAGCGCACTTCGATATCA GAAGTAGCAAAGCCTTGGGT 40.0 50.0 55.9 58.1 1175-1629 bp 0.913043 70.7
rpl32-trnL-UAG_p3 AAAGCGCACTTCGATATCAA GAAGTAGCAAAGCCTTGGGT 40.0 50.0 55.9 58.1 1174-1628 bp 0.913043 70.7
rpl32-trnL-UAG_p4 AAGCGCACTTCGATATCAAA GAAGTAGCAAAGCCTTGGGT 40.0 50.0 55.9 58.1 1173-1627 bp 0.913043 70.7
rpl32-trnL-UAG_p5 AGCGCACTTCGATATCAAAA GAAGTAGCAAAGCCTTGGGT 40.0 50.0 55.9 58.1 1172-1626 bp 0.913043 70.7