Hotspot

ndhF-rpl32

SSC · 117462-118260 · 799 bp

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Pi 0.0629
MarkerSeek score 81.20
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
799
variable_sites
173
pi
0.0629
unique_haplotype_count
23
primer_available
yes
alignment_reliability
0.91
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 ATGAAGGGGAAATTGGGCAG AAAGGACTGTTGGCACTAGA 50.0 45.0 58.1 56.4 1791-2152 bp 0.782609 69.3
ndhF-rpl32_p2 TGTTGCCGTGGGAAAAAGTA AAAGGACTGTTGGCACTAGA 45.0 45.0 57.9 56.4 1149-1510 bp 0.73913 68.2
ndhF-rpl32_p3 AGCAACCGAGCCACAAAATA AAAGGACTGTTGGCACTAGA 45.0 45.0 58.1 56.4 1834-2128 bp 0.608696 62.6
ndhF-rpl32_p4 ATTTAGCAACCGAGCCACAA AAAGGACTGTTGGCACTAGA 45.0 45.0 58.1 56.4 1838-2132 bp 0.608696 62.6
ndhF-rpl32_p5 TAGCAACCGAGCCACAAAAT AAAGGACTGTTGGCACTAGA 45.0 45.0 58.1 56.4 1835-2129 bp 0.608696 62.6