Hotspot

trnP-UGG-psaJ

LSC · 71009-71936 · 928 bp

Back to Indigofera

Pi 0.053

MarkerSeek score 81.10

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 928
variable_sites: 192
pi: 0.053
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.84
markerseek_score: 81.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	670-1101 bp	1.0	90.9
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	669-1100 bp	1.0	90.9
trnP-UGG-psaJ_p3	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	669-1100 bp	1.0	90.9
trnP-UGG-psaJ_p4	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	668-1099 bp	1.0	90.9
trnP-UGG-psaJ_p5	ACAGGATTTGAACCCGTGAC	TGGTGCCACGGAAAGATATG	50.0	50.0	58.1	58.0	681-1112 bp	1.0	90.8