Hotspot

psbK-trnQ-UUG

LSC · 58235-59044 · 810 bp

Back to Indigofera

Pi 0.0738

MarkerSeek score 89.10

Primer available yes

Label psbK-trnQ-UUG

Feature Metadata

label_name: psbK-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 810
variable_sites: 223
pi: 0.0738
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 89.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-trnQ-UUG_p1	CGTAGGCTTCAGGCAATTTC	AATGGATGTGTTCTGGGACG	50.0	50.0	57.2	57.9	800-1092 bp	0.956522	82.7
psbK-trnQ-UUG_p2	GTAGGCTTCAGGCAATTTCG	AATGGATGTGTTCTGGGACG	50.0	50.0	57.2	57.9	799-1091 bp	0.956522	82.7
psbK-trnQ-UUG_p3	TAGGCTTCAGGCAATTTCGC	AATGGATGTGTTCTGGGACG	50.0	50.0	58.9	57.9	798-1090 bp	0.956522	82.1
psbK-trnQ-UUG_p4	GTAGGCTTCAGGCAATTTCG	GACCAAAACCCGTTGCCTTA	50.0	50.0	57.2	58.7	753-1045 bp	1.0	79.9
psbK-trnQ-UUG_p5	GCGTAGGCTTCAGGCAATTT	AATGGATGTGTTCTGGGACG	50.0	50.0	59.2	57.9	801-1093 bp	0.956522	79.8