Hotspot

trnC-GCA-rpoB

LSC · 36456-37685 · 1230 bp

Back to Indigofera

Pi 0.0199

MarkerSeek score 77.40

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1230
variable_sites: 125
pi: 0.0199
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	CTGATTCAACCCGGGTAGTG	50.0	55.0	58.1	58.0	1337-1414 bp	1.0	89.6
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	TCTGATTCAACCCGGGTAGT	50.0	50.0	58.1	58.1	1338-1415 bp	1.0	89.3
trnC-GCA-rpoB_p3	ATTCTCTTGTCGATCAGGCG	CTGATTCAACCCGGGTAGTG	50.0	55.0	57.8	58.0	1361-1438 bp	1.0	88.4
trnC-GCA-rpoB_p4	ATTCTCTTGTCGATCAGGCG	TCTGATTCAACCCGGGTAGT	50.0	50.0	57.8	58.1	1362-1439 bp	1.0	88.1
trnC-GCA-rpoB_p5	CCGGATTTGAACTGGGGAAA	CCTCATTTCTACCCCCAAGC	50.0	55.0	58.1	57.9	1308-1385 bp	0.913043	85.6