Hotspot

psbD-trnT-GGU

LSC · 30207-31659 · 1453 bp

Back to Indigofera

Pi 0.0227

MarkerSeek score 79.70

Primer available yes

Label psbD-trnT-GGU

Feature Metadata

label_name: psbD-trnT-GGU
feature_type: igs
region: LSC
length_bp: 1453
variable_sites: 169
pi: 0.0227
unique_haplotype_count: 23
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 79.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbD-trnT-GGU_p1	CACAAAAACGAAACGGTCCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1443-1676 bp	1.0	90.1
psbD-trnT-GGU_p2	CCACAAAAACGAAACGGTCC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1444-1677 bp	1.0	90.1
psbD-trnT-GGU_p3	CCCACAAAAACGAAACGGTC	GTGGTAGAGTAACGCCATGG	50.0	55.0	58.2	58.1	1445-1678 bp	1.0	90.1
psbD-trnT-GGU_p4	CACAAAAACGAAACGGTCCC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.2	58.1	1446-1679 bp	1.0	90.0
psbD-trnT-GGU_p5	CCACAAAAACGAAACGGTCC	TCAGTGGTAGAGTAACGCCA	50.0	50.0	58.2	58.1	1447-1680 bp	1.0	90.0