Hotspot

trnG-GCC-psbZ

LSC · 26160-26921 · 762 bp

Back to Indigofera

Pi 0.0454

MarkerSeek score 81.90

Primer available yes

Label trnG-GCC-psbZ

Feature Metadata

label_name: trnG-GCC-psbZ
feature_type: igs
region: LSC
length_bp: 762
variable_sites: 169
pi: 0.0454
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 81.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnG-GCC-psbZ_p1	CGTCTTCTCCTTGGCAAAGA	TTTCCGGTACATCCTTATGG	50.0	45.0	57.8	54.5	696-879 bp	0.913043	59.4
trnG-GCC-psbZ_p2	CATAAAAAGGAAGGGGCGGA	TTTCCGGTACATCCTTATGG	50.0	45.0	57.9	54.5	734-915 bp	0.782609	54.4
trnG-GCC-psbZ_p3	ACATAAAAAGGAAGGGGCGG	TTTCCGGTACATCCTTATGG	50.0	45.0	58.2	54.5	735-916 bp	0.782609	54.3
trnG-GCC-psbZ_p4	CATAAAAAGGAAGGGGCGGA	TTTTCCGGTACATCCTTATGG	50.0	42.9	57.9	55.4	735-916 bp	0.782609	53.2
trnG-GCC-psbZ_p5	ACATAAAAAGGAAGGGGCGG	TTTTCCGGTACATCCTTATGG	50.0	42.9	58.2	55.4	736-917 bp	0.782609	53.0