Hotspot

ycf1

IRb · 112357-113652 · 1296 bp

Back to Incarvillea

Pi 0.439

MarkerSeek score 76.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: IRb
length_bp: 1296
variable_sites: 404
pi: 0.439
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GAGCTACTGAGGAACAACGG	TCTCCTTCTGGCGATAGAGG	55.0	55.0	58.0	58.0	1938 bp	0.5	59.9
ycf1_p2	CCCGGAACTTCTTCGTAGTG	TCTCCTTCTGGCGATAGAGG	55.0	55.0	58.0	58.0	1843 bp	0.5	59.8
ycf1_p3	CCGGAACTTCTTCGTAGTGG	TCTCCTTCTGGCGATAGAGG	55.0	55.0	58.0	58.0	1842 bp	0.5	59.8
ycf1_p4	GAGCTACTGAGGAACAACGG	GGGGACGCGTTTCTTCTTAT	55.0	50.0	58.0	58.0	2353 bp	0.5	59.8
ycf1_p5	CCGGAACTTCTTCGTAGTGG	GGGGACGCGTTTCTTCTTAT	55.0	50.0	58.0	58.0	2257 bp	0.5	59.8