Hotspot

ccsA-psaC

SSC · 118940-120921 · 1982 bp

Back to Incarvillea

Pi 0.113

MarkerSeek score 82.40

Primer available yes

Label ccsA-psaC

Feature Metadata

label_name: ccsA-psaC
feature_type: igs
region: SSC
length_bp: 1982
variable_sites: 212
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 82.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-psaC_p1	TGGATATGCTATTTTGGGGTCA	AAGAGATGTGAATCCGCCTG	40.9	50.0	57.2	57.7	2141-2159 bp	1.0	66.9
ccsA-psaC_p2	TGGATATGCTATTTTGGGGTCA	GCCCAACGGATTTCTTGAGT	40.9	50.0	57.2	58.5	2122-2140 bp	1.0	65.8
ccsA-psaC_p3	TGGATATGCTATTTTGGGGTCA	CTGCCCAACGGATTTCTTGA	40.9	50.0	57.2	58.5	2124-2142 bp	1.0	65.8
ccsA-psaC_p4	TGGATATGCTATTTTGGGGTCA	TGCCCAACGGATTTCTTGAG	40.9	50.0	57.2	58.5	2123-2141 bp	1.0	65.8
ccsA-psaC_p5	TGGATATGCTATTTTGGGGTCA	CGAGGACTGTGTTGGTTGTA	40.9	50.0	57.2	57.5	2160-2178 bp	1.0	65.3