Hotspot

rpl32-trnL-UAG

SSC · 116923-117814 · 892 bp

Back to Incarvillea

Pi 0.0955

MarkerSeek score 82.20

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 892
variable_sites: 80
pi: 0.0955
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCGTTAA	TAGGAGATGACATGCCGCTA	50.0	50.0	58.0	58.0	1065-1089 bp	1.0	90.5
rpl32-trnL-UAG_p2	GCCCACATACGACGAAGATT	TAGGAGATGACATGCCGCTA	50.0	50.0	58.1	58.0	1689-1693 bp	1.0	89.9
rpl32-trnL-UAG_p3	GCGCTATTTTGCCGAGTTTT	TAGGAGATGACATGCCGCTA	45.0	50.0	58.0	58.0	1323 bp	0.5	59.8
rpl32-trnL-UAG_p4	GCGCTATTTTGCCGAGTTTT	ACCTAAAAGAGCCGCATAGC	45.0	50.0	58.0	58.0	1850 bp	0.5	59.5
rpl32-trnL-UAG_p5	GCGCTATTTTGCCGAGTTTT	TTTAGGAGATGACATGCCGC	45.0	50.0	58.0	58.0	1325 bp	0.5	59.4