Hotspot

rpl20-rps12

LSC · 71789-72842 · 1054 bp

Back to Incarvillea

Pi 0.172

MarkerSeek score 84.50

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 1054
variable_sites: 164
pi: 0.172
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 84.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CTATCTCGATCGGCGGAAAC	CAGCGTCGAGGAAGATGTAC	55.0	55.0	58.3	58.2	1038-1107 bp	1.0	88.1
rpl20-rps12_p2	CTATCTCGATCGGCGGAAAC	AGCGTCGAGGAAGATGTACT	55.0	50.0	58.3	58.2	1037-1106 bp	1.0	87.5
rpl20-rps12_p3	CTATCTCGATCGGCGGAAAC	TCAGCGTCGAGGAAGATGTA	55.0	50.0	58.3	58.3	1039-1108 bp	1.0	87.5
rpl20-rps12_p4	TTATGCCTATCTCGATCGGC	CAGCGTCGAGGAAGATGTAC	50.0	55.0	57.1	58.2	1044-1113 bp	1.0	83.1
rpl20-rps12_p5	TTATGCCTATCTCGATCGGC	AGCGTCGAGGAAGATGTACT	50.0	50.0	57.1	58.2	1043-1112 bp	1.0	82.5