Hotspot

petA-psbJ

LSC · 65906-66978 · 1073 bp

Back to Incarvillea

Pi 0.0896

MarkerSeek score 82.50

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1073
variable_sites: 90
pi: 0.0896
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 82.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	TCCGTGTCCAAGGTCTTTTG	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	1771-1773 bp	1.0	87.8
petA-psbJ_p2	TCCGTGTCCAAGGTCTTTTG	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	1770-1772 bp	1.0	87.8
petA-psbJ_p3	TCCGATCGTATTTCTCCCGA	AACAGGCCGTTTTGATCCTT	50.0	45.0	58.0	58.0	2261-2263 bp	1.0	86.5
petA-psbJ_p4	TCCGATCGTATTTCTCCCGA	ACAGGCCGTTTTGATCCTTT	50.0	45.0	58.0	58.0	2260-2262 bp	1.0	86.5
petA-psbJ_p5	TCCGATCGTATTTCTCCCGA	TGCGGGTTGGTTATTTGTCA	50.0	45.0	58.0	58.0	2363-2365 bp	1.0	86.1