Hotspot

ycf4-cemA

LSC · 63105-64044 · 940 bp

Back to Incarvillea

Pi 0.116

MarkerSeek score 85.50

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 99
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 85.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTTCGCGT	ACAAAAGAGGAGTGAATGCT	45.0	40.0	58.2	54.9	954-1013 bp	1.0	65.4
ycf4-cemA_p2	GAACAAAAAGCCGCGGAATT	ACAAAAGAGGAGTGAATGCT	45.0	40.0	58.2	54.9	972-1031 bp	1.0	65.0
ycf4-cemA_p3	CGCGTACCAATTGAAGTCCT	ACAAAAGAGGAGTGAATGCT	50.0	40.0	58.3	54.9	939-998 bp	1.0	64.7
ycf4-cemA_p4	TCGCGTACCAATTGAAGTCC	ACAAAAGAGGAGTGAATGCT	50.0	40.0	58.3	54.9	940-999 bp	1.0	64.6
ycf4-cemA_p5	TTGGCCTATTTCTTTCGCGT	ACAAAAGAGGAGTGAATGCTT	45.0	38.1	58.2	55.7	954-1013 bp	1.0	64.1