Hotspot

atpB-rbcL

LSC · 56891-57724 · 834 bp

Back to Incarvillea

Pi 0.113

MarkerSeek score 82.80

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 834
variable_sites: 86
pi: 0.113
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	CGACCTTTCACTACCAGAGC	AGGTTGAGGAGTGACTCGAA	55.0	50.0	58.0	58.0	1098-1166 bp	1.0	91.2
atpB-rbcL_p2	GACCTTTCACTACCAGAGCG	AGGTTGAGGAGTGACTCGAA	55.0	50.0	58.0	58.0	1097-1165 bp	1.0	91.2
atpB-rbcL_p3	GACCTTTCACTACCAGAGCG	AGAAACGATCTCTCCAACGC	55.0	50.0	58.0	58.0	1614-1682 bp	1.0	88.7
atpB-rbcL_p4	CGACCTTTCACTACCAGAGC	AGAAACGATCTCTCCAACGC	55.0	50.0	58.0	58.0	1615-1683 bp	1.0	88.6
atpB-rbcL_p5	GACCTTTCACTACCAGAGCG	GGGGGCCTTGGAAAGTTTTA	55.0	50.0	58.0	58.0	1481 bp	0.5	59.8