Hotspot

psbM-trnD-GUC

LSC · 32391-32995 · 605 bp

Back to Incarvillea

Pi 0.0515
MarkerSeek score 81.00
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
605
variable_sites
30
pi
0.0515
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
81.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGGCGGTAGGAACTAGAATGA TTCAATTGGTCAGAGCACCG 47.6 50.0 58.0 58.5 707-730 bp 1.0 73.9
psbM-trnD-GUC_p2 AGAAAGGCGGTAGGAACTAGA TTCAATTGGTCAGAGCACCG 47.6 50.0 57.9 58.5 711-734 bp 1.0 73.3
psbM-trnD-GUC_p3 AGGCGGTAGGAACTAGAATGA GTTCAATTGGTCAGAGCACC 47.6 50.0 58.0 57.0 708-731 bp 1.0 69.7
psbM-trnD-GUC_p4 GGCGGTAGGAACTAGAATGA TTCAATTGGTCAGAGCACCG 50.0 50.0 56.4 58.5 706-729 bp 1.0 69.6
psbM-trnD-GUC_p5 AGGCGGTAGGAACTAGAATG TTCAATTGGTCAGAGCACCG 50.0 50.0 56.4 58.5 707-730 bp 1.0 69.5