Hotspot

ndhF

SSC · 109061-111280 · 2220 bp

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Pi 0.0451

MarkerSeek score 81.30

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2220
variable_sites: 1096
pi: 0.0451
unique_haplotype_count: 67
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	ACGAGGGGGATTAAACAGGA	AGGGCTAGAATACCCCTTTCT	50.0	47.6	58.0	57.6	2707-2914 bp	0.089552	33.6
ndhF_p2	ACAGGAACAAGAGGGATCCA	AGGGCTAGAATACCCCTTTCT	50.0	47.6	58.0	57.6	2693-2900 bp	0.089552	33.6
ndhF_p3	AAGAGGGATCCACCGAAGAA	AGGGCTAGAATACCCCTTTCT	50.0	47.6	58.0	57.6	2685-2892 bp	0.089552	33.5
ndhF_p4	ACAACTTACGAAACGAGGGG	AGGGCTAGAATACCCCTTTCT	50.0	47.6	57.8	57.6	2719-2926 bp	0.089552	32.6
ndhF_p5	GGTATTGGTCTGGATACGGC	AGGGCTAGAATACCCCTTTCT	55.0	47.6	57.8	57.6	2933-2995 bp	0.059701	30.8