Hotspot

rpl32-trnL-UAG

SSC · 112244-112785 · 542 bp

Back to Impatiens

Pi 0.0838

MarkerSeek score 84.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 542
variable_sites: 345
pi: 0.0838
unique_haplotype_count: 64
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 84.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGGAAAATCCCTTTCAACTGGA	TCCTAAGAGCAGCGTGTCTA	40.9	50.0	57.5	57.9	597-663 bp	0.985075	70.7
rpl32-trnL-UAG_p2	AGGAAAATCCCTTTCAACTGGA	AGCAGCGTGTCTACCAATTT	40.9	45.0	57.5	57.8	590-656 bp	0.985075	70.2
rpl32-trnL-UAG_p3	AGGAAAATCCCTTTCAACTGGA	CCTAAGAGCAGCGTGTCTAC	40.9	55.0	57.5	57.8	596-662 bp	0.985075	70.1
rpl32-trnL-UAG_p4	AGGAAAATCCCTTTCAACTGGA	CTAAGAGCAGCGTGTCTACC	40.9	55.0	57.5	57.8	595-661 bp	0.985075	70.1
rpl32-trnL-UAG_p5	AGGAAAATCCCTTTCAACTGGA	GAGCAGCGTGTCTACCAATT	40.9	50.0	57.5	58.3	591-657 bp	0.985075	69.5