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rpl16-rps3

LSC · 80862-82090 · 1229 bp

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Pi 0.0348

MarkerSeek score 78.10

Primer available yes

Label rpl16-rps3

Feature Metadata

label_name: rpl16-rps3
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 353
pi: 0.0348
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 78.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16-rps3_p1	GTTCAAGTGCCTGAAGACCA	AAATTGCACGCATTGAGTGG	50.0	45.0	58.0	57.9	1339-1496 bp	0.925373	88.2
rpl16-rps3_p2	ACACATCAATTCTCGAGCCC	AAATTGCACGCATTGAGTGG	50.0	45.0	58.0	57.9	1821-1963 bp	0.895522	87.0
rpl16-rps3_p3	GTTCAAGTGCCTGAAGACCA	CACGCATTGAGTGGATCAGA	50.0	50.0	58.0	58.0	1333-1490 bp	0.880597	86.8
rpl16-rps3_p4	ACACATCAATTCTCGAGCCC	CACGCATTGAGTGGATCAGA	50.0	50.0	58.0	58.0	1815-1957 bp	0.850746	85.7
rpl16-rps3_p5	AGATTTTGGACGCCGCTATT	CACGCATTGAGTGGATCAGA	45.0	50.0	58.0	58.0	1679-1751 bp	0.835821	85.0