Hotspot

ycf4-cemA

LSC · 59820-60523 · 704 bp

Back to Impatiens

Pi 0.0466

MarkerSeek score 78.00

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 704
variable_sites: 230
pi: 0.0466
unique_haplotype_count: 65
primer_available: yes
alignment_reliability: 0.91
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGGATCGCGGGTCTTTTTAT	GAAAGAAATCCACCAGGGCA	50.0	50.0	58.0	58.1	659-1316 bp	0.522388	72.0
ycf4-cemA_p2	GTATTTATGCCCGTCGGGTT	GAAAGAAATCCACCAGGGCA	50.0	50.0	58.0	58.1	463-1120 bp	0.522388	71.9
ycf4-cemA_p3	GTCGCATCTTCCTCCGATTT	GAAAGAAATCCACCAGGGCA	50.0	50.0	58.1	58.1	526-1183 bp	0.522388	71.8
ycf4-cemA_p4	TGTGTTTCTATGGGATCGCG	GAAAGAAATCCACCAGGGCA	50.0	50.0	58.1	58.1	670-1327 bp	0.507463	71.2
ycf4-cemA_p5	AAATCGTCGCATCTTCCTCC	GAAAGAAATCCACCAGGGCA	50.0	50.0	58.1	58.1	531-1179 bp	0.462687	69.4