Hotspot

trnT-UGU-trnL-UAA

LSC · 46093-47032 · 940 bp

Back to Impatiens

Pi 0.0668

MarkerSeek score 84.80

Primer available yes

Label trnT-UGU-trnL-UAA

Feature Metadata

label_name: trnT-UGU-trnL-UAA
feature_type: igs
region: LSC
length_bp: 940
variable_sites: 344
pi: 0.0668
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 0.90
markerseek_score: 84.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-UGU-trnL-UAA_p1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	55.0	55.0	57.9	58.2	661-1007 bp	1.0	89.0
trnT-UGU-trnL-UAA_p2	GGATTCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	50.0	55.0	57.8	58.2	693-1039 bp	1.0	88.1
trnT-UGU-trnL-UAA_p3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	55.0	50.0	57.9	57.6	655-1001 bp	1.0	87.7
trnT-UGU-trnL-UAA_p4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	55.0	50.0	57.9	57.5	693-1039 bp	1.0	86.9
trnT-UGU-trnL-UAA_p5	GGATTCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	50.0	50.0	57.8	57.6	687-1033 bp	1.0	86.8