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petN-psbM

LSC · 28074-28888 · 815 bp

Back to Impatiens

Pi 0.0488

MarkerSeek score 83.90

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 815
variable_sites: 274
pi: 0.0488
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	CCCTTTTGACTCTGCGCTAT	GCGCACTGAGCTCTTGATAT	50.0	50.0	58.0	58.1	1684-1763 bp	0.656716	76.9
petN-psbM_p2	TTTTTGTGCGATGTCACTGC	GCGCACTGAGCTCTTGATAT	45.0	50.0	57.9	58.1	1782-1887 bp	0.567164	72.6
petN-psbM_p3	CCCTTTTGACTCTGCGCTAT	AATAGCGCACTGAGCTCTTG	50.0	50.0	58.0	58.4	1744-1762 bp	0.104478	43.0
petN-psbM_p4	CCCTTTTGACTCTGCGCTAT	CAAATAGCGCACTGAGCTCT	50.0	50.0	58.0	58.4	1746-1764 bp	0.104478	43.0
petN-psbM_p5	CCCTTTTGACTCTGCGCTAT	TCAAATAGCGCACTGAGCTC	50.0	50.0	58.0	58.4	1747-1765 bp	0.104478	43.0