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trnC-GCA-petN

LSC · 27181-27983 · 803 bp

Back to Impatiens

Pi 0.0395

MarkerSeek score 81.30

Primer available yes

Label trnC-GCA-petN

Feature Metadata

label_name: trnC-GCA-petN
feature_type: igs
region: LSC
length_bp: 803
variable_sites: 259
pi: 0.0395
unique_haplotype_count: 63
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-petN_p1	GGGTGTCGCCTGATTAACAA	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.2	57.6	682-947 bp	0.955224	85.9
trnC-GCA-petN_p2	AGTTCAAATCTGGGTGTCGC	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.5	57.6	534-958 bp	1.0	85.4
trnC-GCA-petN_p3	GGGTGTCGCCTGATTAACAA	TACCATTAAAGCAGCCCAGG	50.0	50.0	58.2	57.6	633-898 bp	0.955224	85.3
trnC-GCA-petN_p4	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	50.0	50.0	58.4	57.6	559-983 bp	0.970149	85.2
trnC-GCA-petN_p5	GGGGGACTGCAAATCCTTTT	TACCATTAAAGCAGCCCAGG	50.0	50.0	58.4	57.6	510-934 bp	0.970149	84.5