Hotspot

atpH-atpI

LSC · 12536-13695 · 1160 bp

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Pi 0.0465

MarkerSeek score 89.30

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1160
variable_sites: 361
pi: 0.0465
unique_haplotype_count: 64
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 89.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	CAGCAGCAATAACAGAAGCG	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	969-1270 bp	1.0	89.6
atpH-atpI_p2	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.8	57.9	976-1277 bp	1.0	89.2
atpH-atpI_p3	AAGCGGCAGAAATAAGTGGA	TTTTGCAACTTTAGCTGCGG	45.0	45.0	57.5	57.9	954-1255 bp	1.0	87.0
atpH-atpI_p4	CGCGATACCTTCTACAGCTT	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.5	57.9	1049-1350 bp	1.0	87.0
atpH-atpI_p5	CCAGCAGCAATAACAGAAGC	TTTTGCAACTTTAGCTGCGG	50.0	45.0	57.4	57.9	970-1271 bp	1.0	86.5