Hotspot

matK-trnK-UUU

LSC · 2706-4223 · 1518 bp

Back to Impatiens

Pi 0.0392

MarkerSeek score 81.30

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 1518
variable_sites: 485
pi: 0.0392
unique_haplotype_count: 66
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TCCTTGATACCTAACATAATGCA	AACGGTAGAGTACTCGGCTT	34.8	50.0	55.2	58.2	1544-1577 bp	0.970149	50.8
matK-trnK-UUU_p2	TCCTTGATACCTAACATAATGCA	ACGGTAGAGTACTCGGCTTT	34.8	50.0	55.2	58.2	1543-1576 bp	0.970149	50.8
matK-trnK-UUU_p3	TTCCTTGATACCTAACATAATGCA	AACGGTAGAGTACTCGGCTT	33.3	50.0	55.9	58.2	1545-1578 bp	0.970149	50.8
matK-trnK-UUU_p4	TTCCTTGATACCTAACATAATGCA	ACGGTAGAGTACTCGGCTTT	33.3	50.0	55.9	58.2	1544-1577 bp	0.970149	50.8
matK-trnK-UUU_p5	TCCTTGATACCTAACATAATGCA	GGGTTGCTAACTCAACGGTA	34.8	50.0	55.2	57.5	1557-1590 bp	0.925373	49.0