primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	matK-trnK-UUU	1	TCCTTGATACCTAACATAATGCA	AACGGTAGAGTACTCGGCTT	23	20	34.8	50.0	55.2	58.2	0.0	17.1	5.982162	2706	4223	1544-1577	0.970149	50.8
matK-trnK-UUU_p2	matK-trnK-UUU	2	TCCTTGATACCTAACATAATGCA	ACGGTAGAGTACTCGGCTTT	23	20	34.8	50.0	55.2	58.2	0.0	17.1	5.982162	2706	4223	1543-1576	0.970149	50.8
matK-trnK-UUU_p3	matK-trnK-UUU	3	TTCCTTGATACCTAACATAATGCA	AACGGTAGAGTACTCGGCTT	24	20	33.3	50.0	55.9	58.2	0.0	17.1	6.260046	2706	4223	1545-1578	0.970149	50.8
matK-trnK-UUU_p4	matK-trnK-UUU	4	TTCCTTGATACCTAACATAATGCA	ACGGTAGAGTACTCGGCTTT	24	20	33.3	50.0	55.9	58.2	0.0	17.1	6.260046	2706	4223	1544-1577	0.970149	50.8
matK-trnK-UUU_p5	matK-trnK-UUU	5	TCCTTGATACCTAACATAATGCA	GGGTTGCTAACTCAACGGTA	23	20	34.8	50.0	55.2	57.5	0.0	0.0	6.274500	2706	4223	1557-1590	0.925373	49.0
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	CGTTCCGCAACTGGTACTAA	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.077079	4261	4926	1614-1724	0.925373	88.4
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	TCAGGATCAGTCGTGGTCTT	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.083511	4261	4926	1343-1455	0.925373	88.3
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	CCTTTCAGGATCAGTCGTGG	AAAAGGGTATGTTGCTGCCA	20	20	55.0	45.0	58.0	58.0	0.0	0.0	0.023978	4261	4926	1347-1459	0.850746	85.8
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	CGAATCCTCGCTTCATCCAA	AAAAGGGTATGTTGCTGCCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.068268	4261	4926	1300-1412	0.820896	84.3
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	CCTTTCAGGATCAGTCGTGG	TAACTGCCCCAGAATGAGGA	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.050322	4261	4926	1225-1339	0.701493	79.7
atpH-atpI_p1	atpH-atpI	1	CAGCAGCAATAACAGAAGCG	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.302403	12536	13695	969-1270	1.000000	89.6
atpH-atpI_p2	atpH-atpI	2	GCCAATCCAGCAGCAATAAC	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.347127	12536	13695	976-1277	1.000000	89.2
atpH-atpI_p3	atpH-atpI	3	AAGCGGCAGAAATAAGTGGA	TTTTGCAACTTTAGCTGCGG	20	20	45.0	45.0	57.5	57.9	1.4	0.0	0.622679	12536	13695	954-1255	1.000000	87.0
atpH-atpI_p4	atpH-atpI	4	CGCGATACCTTCTACAGCTT	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.5	57.9	0.0	0.0	0.626453	12536	13695	1049-1350	1.000000	87.0
atpH-atpI_p5	atpH-atpI	5	CCAGCAGCAATAACAGAAGC	TTTTGCAACTTTAGCTGCGG	20	20	50.0	45.0	57.4	57.9	0.0	0.0	0.689275	12536	13695	970-1271	1.000000	86.5
rpoB-trnC-GCA_p1	rpoB-trnC-GCA	1	TTTGTTCTTGCATGTCCCGA	GCAGTGACATCGCACAAAAA	20	20	45.0	45.0	58.0	57.9	0.0	0.0	0.143963	26012	27099	1533-2146	0.880597	86.1
rpoB-trnC-GCA_p2	rpoB-trnC-GCA	2	CGAGCCCATATCCTTGCTTT	GCAGTGACATCGCACAAAAA	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.145356	26012	27099	1753-2366	0.880597	86.1
rpoB-trnC-GCA_p3	rpoB-trnC-GCA	3	TTTGTTCTTGCATGTCCCGA	CTTTTGGAAACACGCAGACC	20	20	45.0	50.0	58.0	57.9	0.0	0.0	0.145406	26012	27099	1404-1656	0.880597	86.1
rpoB-trnC-GCA_p4	rpoB-trnC-GCA	4	GAAAATACCGGATCCCCACC	CTTTTGGAAACACGCAGACC	20	20	55.0	50.0	58.0	57.9	9.5	0.0	0.142615	26012	27099	1831-2083	0.835821	84.3
rpoB-trnC-GCA_p5	rpoB-trnC-GCA	5	GAAAATACCGGATCCCCACC	GCAGTGACATCGCACAAAAA	20	20	55.0	45.0	58.0	57.9	9.5	0.0	0.141172	26012	27099	2218-2573	0.820896	83.7
trnC-GCA-petN_p1	trnC-GCA-petN	1	GGGTGTCGCCTGATTAACAA	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.2	57.6	1.7	0.0	0.534788	27181	27983	682-947	0.955224	85.9
trnC-GCA-petN_p2	trnC-GCA-petN	2	AGTTCAAATCTGGGTGTCGC	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.5	57.6	0.0	0.0	0.827105	27181	27983	534-958	1.000000	85.4
trnC-GCA-petN_p3	trnC-GCA-petN	3	GGGTGTCGCCTGATTAACAA	TACCATTAAAGCAGCCCAGG	20	20	50.0	50.0	58.2	57.6	1.7	0.0	0.619298	27181	27983	633-898	0.955224	85.3
trnC-GCA-petN_p4	trnC-GCA-petN	4	GGGGGACTGCAAATCCTTTT	AGAGTCCACTTCTTCCCCAT	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.706353	27181	27983	559-983	0.970149	85.2
trnC-GCA-petN_p5	trnC-GCA-petN	5	GGGGGACTGCAAATCCTTTT	TACCATTAAAGCAGCCCAGG	20	20	50.0	50.0	58.4	57.6	14.7	0.0	0.790863	27181	27983	510-934	0.970149	84.5
petN-psbM_p1	petN-psbM	1	CCCTTTTGACTCTGCGCTAT	GCGCACTGAGCTCTTGATAT	20	20	50.0	50.0	58.0	58.1	9.7	16.2	0.166571	28074	28888	1684-1763	0.656716	76.9
petN-psbM_p2	petN-psbM	2	TTTTTGTGCGATGTCACTGC	GCGCACTGAGCTCTTGATAT	20	20	45.0	50.0	57.9	58.1	0.0	16.2	0.254961	28074	28888	1782-1887	0.567164	72.6
petN-psbM_p3	petN-psbM	3	CCCTTTTGACTCTGCGCTAT	AATAGCGCACTGAGCTCTTG	20	20	50.0	50.0	58.0	58.4	9.7	16.2	0.380175	28074	28888	1744-1762	0.104478	43.0
petN-psbM_p4	petN-psbM	4	CCCTTTTGACTCTGCGCTAT	CAAATAGCGCACTGAGCTCT	20	20	50.0	50.0	58.0	58.4	9.7	16.2	0.380175	28074	28888	1746-1764	0.104478	43.0
petN-psbM_p5	petN-psbM	5	CCCTTTTGACTCTGCGCTAT	TCAAATAGCGCACTGAGCTC	20	20	50.0	50.0	58.0	58.4	9.7	11.5	0.384804	28074	28888	1747-1765	0.104478	43.0
trnT-UGU-trnL-UAA_p1	trnT-UGU-trnL-UAA	1	GCGATGCTCTAACCTCTGAG	CCGTAGCGTCTACCGATTTC	20	20	55.0	55.0	57.9	58.2	0.0	10.2	0.381189	46093	47032	661-1007	1.000000	89.0
trnT-UGU-trnL-UAA_p2	trnT-UGU-trnL-UAA	2	GGATTCGAACCGATGACCAT	CCGTAGCGTCTACCGATTTC	20	20	50.0	55.0	57.8	58.2	0.0	10.2	0.488942	46093	47032	693-1039	1.000000	88.1
trnT-UGU-trnL-UAA_p3	trnT-UGU-trnL-UAA	3	GCGATGCTCTAACCTCTGAG	CGTCTACCGATTTCGCCATA	20	20	55.0	50.0	57.9	57.6	0.0	0.0	0.543526	46093	47032	655-1001	1.000000	87.7
trnT-UGU-trnL-UAA_p4	trnT-UGU-trnL-UAA	4	GCGATGCTCTAACCTCTGAG	GGTTTCCATACCAAGGCTCA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.640147	46093	47032	693-1039	1.000000	86.9
trnT-UGU-trnL-UAA_p5	trnT-UGU-trnL-UAA	5	GGATTCGAACCGATGACCAT	CGTCTACCGATTTCGCCATA	20	20	50.0	50.0	57.8	57.6	0.0	0.0	0.651279	46093	47032	687-1033	1.000000	86.8
ndhC-trnV-UAC_p1	ndhC-trnV-UAC	1	TGGTTCAGGCATTTGCTCAT	TGGTAGTGCTCCTGGATCAA	20	20	45.0	50.0	58.1	58.0	2.1	1.1	0.121303	50402	50844	1167-1490	0.955224	89.2
ndhC-trnV-UAC_p2	ndhC-trnV-UAC	2	TGGTTCAGGCATTTGCTCAT	TTGGTAGTGCTCCTGGATCA	20	20	45.0	50.0	58.1	58.0	2.1	0.0	0.121303	50402	50844	1168-1491	0.955224	89.2
ndhC-trnV-UAC_p3	ndhC-trnV-UAC	3	TGGTTCAGGCATTTGCTCAT	CCGAGAAAGTCTACGGTTCG	20	20	45.0	55.0	58.1	58.1	2.1	0.0	0.180045	50402	50844	967-1283	0.940299	88.2
ndhC-trnV-UAC_p4	ndhC-trnV-UAC	4	TGGTTCAGGCATTTGCTCAT	CCGATTCAGGTGCCCATTTA	20	20	45.0	50.0	58.1	57.9	2.1	0.0	0.123847	50402	50844	1438-1760	0.910448	87.4
ndhC-trnV-UAC_p5	ndhC-trnV-UAC	5	GGGGCTAAAACTCCGGAAAT	TGGTAGTGCTCCTGGATCAA	20	20	50.0	50.0	57.9	58.0	5.2	1.1	0.188114	50402	50844	560-881	0.835821	83.9
ycf4-cemA_p1	ycf4-cemA	1	GGGATCGCGGGTCTTTTTAT	GAAAGAAATCCACCAGGGCA	20	20	50.0	50.0	58.0	58.1	6.1	0.0	0.113472	59820	60523	659-1316	0.522388	72.0
ycf4-cemA_p2	ycf4-cemA	2	GTATTTATGCCCGTCGGGTT	GAAAGAAATCCACCAGGGCA	20	20	50.0	50.0	58.0	58.1	30.6	0.0	0.118976	59820	60523	463-1120	0.522388	71.9
ycf4-cemA_p3	ycf4-cemA	3	GTCGCATCTTCCTCCGATTT	GAAAGAAATCCACCAGGGCA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.135319	59820	60523	526-1183	0.522388	71.8
ycf4-cemA_p4	ycf4-cemA	4	TGTGTTTCTATGGGATCGCG	GAAAGAAATCCACCAGGGCA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.142056	59820	60523	670-1327	0.507463	71.2
ycf4-cemA_p5	ycf4-cemA	5	AAATCGTCGCATCTTCCTCC	GAAAGAAATCCACCAGGGCA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.135319	59820	60523	531-1179	0.462687	69.4
rpl16-rps3_p1	rpl16-rps3	1	GTTCAAGTGCCTGAAGACCA	AAATTGCACGCATTGAGTGG	20	20	50.0	45.0	58.0	57.9	0.0	20.0	0.101518	80862	82090	1339-1496	0.925373	88.2
rpl16-rps3_p2	rpl16-rps3	2	ACACATCAATTCTCGAGCCC	AAATTGCACGCATTGAGTGG	20	20	50.0	45.0	58.0	57.9	7.8	20.0	0.098675	80862	82090	1821-1963	0.895522	87.0
rpl16-rps3_p3	rpl16-rps3	3	GTTCAAGTGCCTGAAGACCA	CACGCATTGAGTGGATCAGA	20	20	50.0	50.0	58.0	58.0	0.0	12.2	0.047017	80862	82090	1333-1490	0.880597	86.8
rpl16-rps3_p4	rpl16-rps3	4	ACACATCAATTCTCGAGCCC	CACGCATTGAGTGGATCAGA	20	20	50.0	50.0	58.0	58.0	7.8	12.2	0.044174	80862	82090	1815-1957	0.850746	85.7
rpl16-rps3_p5	rpl16-rps3	5	AGATTTTGGACGCCGCTATT	CACGCATTGAGTGGATCAGA	20	20	45.0	50.0	58.0	58.0	0.0	12.2	0.052722	80862	82090	1679-1751	0.835821	85.0
ndhF_p1	ndhF	1	ACGAGGGGGATTAAACAGGA	AGGGCTAGAATACCCCTTTCT	20	21	50.0	47.6	58.0	57.6	0.0	0.0	1.478684	109061	111280	2707-2914	0.089552	33.6
ndhF_p2	ndhF	2	ACAGGAACAAGAGGGATCCA	AGGGCTAGAATACCCCTTTCT	20	21	50.0	47.6	58.0	57.6	15.2	0.0	1.484610	109061	111280	2693-2900	0.089552	33.6
ndhF_p3	ndhF	3	AAGAGGGATCCACCGAAGAA	AGGGCTAGAATACCCCTTTCT	20	21	50.0	47.6	58.0	57.6	15.2	0.0	1.487196	109061	111280	2685-2892	0.089552	33.5
ndhF_p4	ndhF	4	ACAACTTACGAAACGAGGGG	AGGGCTAGAATACCCCTTTCT	20	21	50.0	47.6	57.8	57.6	0.0	0.0	1.599144	109061	111280	2719-2926	0.089552	32.6
ndhF_p5	ndhF	5	GGTATTGGTCTGGATACGGC	AGGGCTAGAATACCCCTTTCT	20	21	55.0	47.6	57.8	57.6	0.0	0.0	1.605136	109061	111280	2933-2995	0.059701	30.8
ndhF-rpl32_p1	ndhF-rpl32	1	CAGGCCGTGTAAACCAAAAC	AGGCTTTTAACGCTGTCCAA	20	20	50.0	45.0	57.9	58.0	0.0	0.0	0.152708	111281	112093	952-1418	0.925373	87.8
ndhF-rpl32_p2	ndhF-rpl32	2	CAGGCCGTGTAAACCAAAAC	TTCACCATAGCGGCTTTCTC	20	20	50.0	50.0	57.9	58.0	0.0	8.3	0.152543	111281	112093	1964-2040	0.208955	59.1
ndhF-rpl32_p3	ndhF-rpl32	3	GGCAAATAATGGAACGGCAC	AGGCTTTTAACGCTGTCCAA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.103097	111281	112093	1597-1612	0.074627	44.4
ndhF-rpl32_p4	ndhF-rpl32	4	GGCAAATAATGGAACGGCAC	AAGGCTTTTAACGCTGTCCA	20	20	50.0	45.0	58.1	58.0	0.0	0.0	0.103097	111281	112093	1598-1613	0.074627	44.3
ndhF-rpl32_p5	ndhF-rpl32	5	GGCAAATAATGGAACGGCAC	TTCACCATAGCGGCTTTCTC	20	20	50.0	50.0	58.1	58.0	0.0	8.3	0.102932	111281	112093	2219-2234	0.059701	43.4
rpl32-trnL-UAG_p1	rpl32-trnL-UAG	1	AGGAAAATCCCTTTCAACTGGA	TCCTAAGAGCAGCGTGTCTA	22	20	40.9	50.0	57.5	57.9	0.2	0.0	2.593746	112244	112785	597-663	0.985075	70.7
rpl32-trnL-UAG_p2	rpl32-trnL-UAG	2	AGGAAAATCCCTTTCAACTGGA	AGCAGCGTGTCTACCAATTT	22	20	40.9	45.0	57.5	57.8	0.2	0.0	2.654473	112244	112785	590-656	0.985075	70.2
rpl32-trnL-UAG_p3	rpl32-trnL-UAG	3	AGGAAAATCCCTTTCAACTGGA	CCTAAGAGCAGCGTGTCTAC	22	20	40.9	55.0	57.5	57.8	0.2	0.0	2.665764	112244	112785	596-662	0.985075	70.1
rpl32-trnL-UAG_p4	rpl32-trnL-UAG	4	AGGAAAATCCCTTTCAACTGGA	CTAAGAGCAGCGTGTCTACC	22	20	40.9	55.0	57.5	57.8	0.2	0.0	2.665764	112244	112785	595-661	0.985075	70.1
rpl32-trnL-UAG_p5	rpl32-trnL-UAG	5	AGGAAAATCCCTTTCAACTGGA	GAGCAGCGTGTCTACCAATT	22	20	40.9	50.0	57.5	58.3	0.2	0.0	2.742615	112244	112785	591-657	0.985075	69.5
ycf1_p1	ycf1	1	GCAAGTGGAATGCTATCCCA	GCGCCCAAAGGAAGAAAATC	20	20	50.0	50.0	57.9	58.0	6.0	0.0	0.060846	125646	125765	277-304	0.253731	61.7
ycf1_p2	ycf1	2	AGTTCAGTCTCCATTCCCCA	GCGCCCAAAGGAAGAAAATC	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.048501	125646	125765	646-673	0.208955	60.0
ycf1_p3	ycf1	3	AGTTCAGTCTCCATTCCCCA	GAAGAAGTGCAAGTGGGACA	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.077474	125646	125765	836-863	0.119403	54.0
ycf1_p4	ycf1	4	TCTGCTATTTCTATCCGCGC	GCGCCCAAAGGAAGAAAATC	20	20	50.0	50.0	57.9	58.0	0.0	0.0	0.069701	125646	125765	280-280	0.089552	44.2
ycf1_p5	ycf1	5	CTGCTATTTCTATCCGCGCT	GCGCCCAAAGGAAGAAAATC	20	20	50.0	50.0	57.9	58.0	5.6	0.0	0.075397	125646	125765	279-279	0.089552	44.1