Hotspot

ycf1

SSC · 125712-131228 · 5517 bp

Back to Illigera

Pi 0.0118

MarkerSeek score 49.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5517
variable_sites: 97
pi: 0.0118
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AGTTGCGCATTAACCTTGGA	TGACGGATCTGTCCAATAGGA	45.0	47.6	58.1	58.0	274-283 bp	1.0	73.6
ycf1_p2	AGTTGCGCATTAACCTTGGA	TGACGGATCTGTCCAATAGG	45.0	50.0	58.1	56.4	274-283 bp	1.0	69.2
ycf1_p3	GTTGCGCATTAACCTTGGAA	TGACGGATCTGTCCAATAGGA	45.0	47.6	57.3	58.0	273-282 bp	1.0	68.6
ycf1_p4	AAGTTGCGCATTAACCTTGG	TGACGGATCTGTCCAATAGGA	45.0	47.6	57.3	58.0	275-284 bp	1.0	68.6
ycf1_p5	AGTTGCGCATTAACCTTGGA	GACGGATCTGTCCAATAGGA	45.0	50.0	58.1	56.1	273-282 bp	1.0	66.9