Hotspot

rpl23-rpl2

IRa · 154662-154888 · 227 bp

Back to Illigera

Pi 0.0151

MarkerSeek score 54.00

Primer available yes

Label rpl23-rpl2

Feature Metadata

label_name: rpl23-rpl2
feature_type: igs
region: IRa
length_bp: 227
variable_sites: 4
pi: 0.0151
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-rpl2_p1	CGAATCAGGATCTAATAGGACGG	TTTCGCTTGACTGTCTACGG	47.8	50.0	58.0	57.9	311-325 bp	1.0	60.4
rpl23-rpl2_p2	CGAATCAGGATCTAATAGGACGG	GACTGTCTACGGCTCCATTG	47.8	55.0	58.0	58.1	303-317 bp	1.0	60.4
rpl23-rpl2_p3	CGAATCAGGATCTAATAGGACGG	TCGTGGATTGGATTTCGCTT	47.8	45.0	58.0	57.9	323-337 bp	1.0	59.9
rpl23-rpl2_p4	CGAATCAGGATCTAATAGGACGG	TTCGTGGATTGGATTTCGCT	47.8	45.0	58.0	57.9	324-338 bp	1.0	59.9
rpl23-rpl2_p5	CGAATCAGGATCTAATAGGACGG	CGTGGATTGGATTTCGCTTG	47.8	50.0	58.0	57.8	322-336 bp	1.0	59.3