Hotspot

ndhF-rpl32

SSC · 113711-114792 · 1082 bp

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Pi 0.0186

MarkerSeek score 62.10

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 1082
variable_sites: 30
pi: 0.0186
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCGAAAGGTATGATCCATGC	GCTGCAGCTTGATATCCCTT	50.0	50.0	57.0	58.0	1206-1222 bp	1.0	74.6
ndhF-rpl32_p2	CTGGAAGTGAAGCGAAAGGTA	GCTGCAGCTTGATATCCCTT	47.6	50.0	58.0	58.0	1217-1233 bp	1.0	74.1
ndhF-rpl32_p3	TGGAAGTGAAGCGAAAGGTA	GCTGCAGCTTGATATCCCTT	45.0	50.0	56.8	58.0	1216-1232 bp	1.0	72.7
ndhF-rpl32_p4	GCGAAAGGTATGATCCATGC	TTGCTGCAGCTTGATATCCC	50.0	50.0	57.0	58.3	1208-1224 bp	1.0	72.2
ndhF-rpl32_p5	CTGGAAGTGAAGCGAAAGGTA	TTGCTGCAGCTTGATATCCC	47.6	50.0	58.0	58.3	1219-1235 bp	1.0	71.7