Hotspot

trnR-ACG-trnN-GUU

IRb · 109545-110115 · 571 bp

Back to Illigera

Pi 0.0108

MarkerSeek score 67.40

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 571
variable_sites: 9
pi: 0.0108
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 67.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	CAGAGGATTAGAGCACGTGG	GCTGTTAACTGACTGGTCGT	55.0	50.0	57.8	57.8	676-685 bp	1.0	79.9
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	TGGTCGTAGGTTCGAATCCT	55.0	50.0	57.8	58.2	663-672 bp	1.0	79.9
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	CTGGTCGTAGGTTCGAATCC	55.0	55.0	57.8	57.8	664-673 bp	1.0	79.5
trnR-ACG-trnN-GUU_p4	CAGAGGATTAGAGCACGTGG	ATCCCCCGTTATTCCTCAGT	55.0	50.0	57.8	57.8	717-726 bp	1.0	79.4
trnR-ACG-trnN-GUU_p5	CAGAGGATTAGAGCACGTGG	CGGGAGTTGCACTCTATGAG	55.0	55.0	57.8	57.8	742-751 bp	1.0	79.1