Hotspot

rpl23-trnI-CAU

IRb · 87227-87401 · 175 bp

Back to Illigera

Pi 0.061

MarkerSeek score 64.40

Primer available yes

Label rpl23-trnI-CAU

Feature Metadata

label_name: rpl23-trnI-CAU
feature_type: igs
region: IRb
length_bp: 175
variable_sites: 16
pi: 0.061
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl23-trnI-CAU_p1	TGTTCCCCAATAACCGAATACT	GAATGGTTAAAGCGCCCAAC	40.9	50.0	57.2	58.0	295 bp	1.0	61.5
rpl23-trnI-CAU_p2	GTTCCCCAATAACCGAATACTCT	GAATGGTTAAAGCGCCCAAC	43.5	50.0	58.0	58.0	294 bp	1.0	60.0
rpl23-trnI-CAU_p3	ATTGTTCCCCAATAACCGAA	GAATGGTTAAAGCGCCCAAC	40.0	50.0	54.9	58.0	297 bp	1.0	59.5
rpl23-trnI-CAU_p4	TTGTTCCCCAATAACCGAAT	GAATGGTTAAAGCGCCCAAC	40.0	50.0	54.9	58.0	296 bp	1.0	59.5
rpl23-trnI-CAU_p5	TGTTCCCCAATAACCGAATACT	TTCGCGATGTACAAGGATCC	40.9	50.0	57.2	57.8	334 bp	1.0	59.3